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Ramos also emphasizes inputs many clinicians overlook: early-life colonization — whether a baby was born vaginally or by cesarean, and early antibiotic exposure — shapes the microbiome for years, along with hormonal fluctuations across the lifespan, alcohol, bile-acid imbalances, and aging-related changes

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Non-senescent (2000 cells per well) and senescent (5000 cells/well) IMR90 fibroblasts were then plated in 150 μl medium