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To determine CI activity, MAS plus alamethicin (2.5 μg ml −1 ) and cytochrome c (10 μg ml −1 ) were added to the mitochondria followed by 5 mM pyruvate + 5 mM malate + 1 mM NADH at port A, 2 μM rotenone + 4 μM antimycin A at port B, 0.5 mM TMPD + 1 mM ascorbic acid at port C and 50 mM azide at port D