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📌 Column Specifications • Chiral Stationary Phase (CSP): Cellulose tris(3,5-dimethylphenylcarbamate) coated on high-purity silica gel • Typical Column Dimension: 250 × 4.6 mm, 5 µm • Operating Mode: Primarily Normal-Phase Chromatography ⚙️ Principle of Chiral Recognition Enantiomers interact differently with the chiral stationary phase through a combination of: ✔ Hydrogen bonding ✔ π–π interactions ✔ Dipole–dipole interactions ✔ Steric (three-dimensional) recognition ✔ Van der Waals forces These multiple interactions create different retention times for each enantiomer, enabling effective chiral separation