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After 19 h incubation in a humidified CO 2 /air incubator, the ring-enriched fraction was separated on and collected from a discontinuous 40/80/90% Percoll gradient, containing mannitol (6% wt/vol)

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Signalling Paradox In macrophages, the inhibitory effect of sulforaphane on cytokine expression and M1 markers’ C-C motif chemokine receptor 7, IL-23, and iNOS has been shown to be associated with the inhibition of mitogen-activated protein kinases (MAPK), p38, c-Jun N-terminal kinase (JNK), and phosphorylation [99]